Effects of Flavonoid Fractions from Calendula officinalis Flowers in Parent and Tamoxifen Resistant T47D Human Breast Cancer Cells Effects of flavonoids in human breast cancer cells
Iranian Journal of Pharmaceutical Sciences,
Vol. 1 No. 3 (2005),
1 July 2005
,
Page 161-166
https://doi.org/10.22037/ijps.v1.39492
Abstract
Three major flavonoid fractions were separated from a methanol extract of Calendula officinalis flowers by preparative TLC. These fractions were evaluated for the inhibition of parent and tamoxifen resistant T47D human breast cancer cells. We also examined the effect of quercetin and isorhamnetin on the growth of parent and resistant T47D cells in the presence and absence of tamoxifen. It was found
that quercetin increased cell proliferation of the resistant T47D cells at the presence of tamoxifen but no effect was detected by using quercitin alone. The fractions isolated from Calendula officinalis did not show any inhibitory effects on the cells.Isorhamnetin did not have any proliferative or anti-proliferative activity on the both cell lines.
Keywords:
- Calendula officinalis
- Flavonoid; Isorhamnetin
- T47D cells
- Quercetin
How to Cite
Ostad, S. N. ., Monsef-Esfahani, H. R. ., Taheri, S. ., Azizi, E. ., & Faramarzi, M. A. (2005). Effects of Flavonoid Fractions from Calendula officinalis Flowers in Parent and Tamoxifen Resistant T47D Human Breast Cancer Cells: Effects of flavonoids in human breast cancer cells. Iranian Journal of Pharmaceutical Sciences, 1(3), 161–166. https://doi.org/10.22037/ijps.v1.39492
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[28] Santen RJ. Inhibition of aromatase: insights from recent studies. Steroids 2003; 68: 559-67.
[2] Chen S. Aromatase and breast cancer. Front Biosci 1998; 3: 922-33.
[3] Howe GR, Hirohata T, Hislop TG, Iscovich JM, Yuan JM, Katsouyanni K, Lupin F, Marubini E, Modan B, Rahan T, Toniolo P, Shunzhang Y. Dietary factors and risk of breast cancer: combined analysis of 12-case-control studies. J Natl Cancer Instit 1990; 82: 561-9.
[4] Vrijsen R, Everaert L, Boeye A. Antiviral activity of flavones and potentiation by ascorbate. J General Virol 1998; 69: 1749-51.
[5] Verma A. Inhibition of 7, 12-dimethyl benz(a)anthracene and N-nitroso methyl urea induced rat mammary cancer by dietary flavonol quercetin. Cancer Res 1998; 48: 5454-8.
[6] Avila MA, Velasco JA, Cansado J, Notario V. Quercetin mediates the down regulation of mutant p53 in the breast cancer cell line MDA-MB 468. Cancer Res 1994; 54: 2424-8.
[7] Amirghofran Z, Azadbakht M, Karimi M H. Evaluation of the immunomodulatory effects of five herbal plants. J Ethnopharmacol 2000; 72: 167-72.
[8] Elias R. Antimutagenic activity of some saponins isolated from Calendula officinalis L., C. arvensis L. and Hedera helix L. Mutagenesis 1990; 5: 327-31.
[9] Kalvatchev Z, Walder R, Garzaro D. Anti-HIV activity of extracts from Calendula officinalis flowers. Biomed Pharmaclo Ther 1997; 51: 176-80.
[10] Raynaud J. Cytotoxic and antitumoral activity of Calendula officinalis extracts. Pharmazie 1998; 43: 220-1.
[11] Andersen FA. Final report on the safety assessment of Calendula officinalis extract and Calendula officinais. Internat J Toxicol 2001; 20 (suppl): 13-20.
[12] Masterova I, Grancaiova Z, Uhrinova S, Suchy V, Ubik K, Nagy M. Flavonoids in flowers of Calendula officinalis L. Chem Paper 1991; 45: 105-8.
[13] Miranda CL, Stevens JF, Helmrich A, Henderson MC, Rodriguez RJ, Yung YH, Deinzer ML, Barnes DW, Buhler DR. Antiproliferative and cytotoxic effects of prenylated flavonoids from HOPS (Humulns lupulus) in human cancer cell lines. Food Chem Toxicol 1999; 37: 271-85.
[14] Le Bail JC, Varnat F, Nicolas JC, Habiroux G. Estrogenic and antiproliferative activities on MCF-7 human breast cancer cells by flavonoids, Cancer Lett 1998; 130: 209-16.
[15] Le Bail JC, Champavier Y, Chulia AJ, Habrioux G. Effects of phytoestogens on aromatase 3-beta and 17-beta hydroxysteroid dehydrogenase activities and human breast cancer cells. Life Sci 2000; 66: 1281-91.
[16] Collins MB, Mclachlan JA, Arnold SF. The estrogenic and antiestrogenic activities of phytochemical with the human estrogen receptor expressed in yeast. Steroids 1997; 62: 365-72.
[17] Ibrahim AR, Abul-Haji YJ. Aromatase inhibition by flavonoids. J Steroid Biochem Mol Biol 1990; 37: 257-60.
[18] Kao YC, Zhou C, Sherman M, Laughton CA, Chen S. Molecular basis of the inhibition of human aromatase (estrogen synthetase) by flavone and isoflavone phytoestrogens: a site-directed mutagenesis study. Environ Health Perspect 1998; 106: 85-92.
[19] Campell DR, Kurzer MS. Flavonoid inhibition of aromatase enzyme activity in human preadipocytes. J Steroid Biochem Mol Biol 1993; 46: 381-8.
[20] Kellis JT, Vickery LE. Inhibition of human estrogen synthetase (aromatase) by flavones. Science 1984; 225: 1032-4.
[21] Miksicek RJ. Esterogenic flavonoids: structural requirements for biological activity PSEBM 1995; 208: 44-50.
[22] Saarinen N, Joshi SC, Ahotupa M, Li X, Ammala J, Makela S, Ritso S. No evidence for the in vivo activity of aromatase-inhibiting flavonoids. J Steroid Biochem Mol Biol 2001; 78: 231-9.
[23] Makela S, Poutanen M, Kostian ML, Lehtimaki N, Strauss L, Santti R, Vihko R. Inhibition of 17b-hydroxysteroid oxidoreductase by flavonoids in breast and prostate cancer cells. PSEBM 1998; 217: 310-6.
[24] Verma SP, Salamon E, Goldin B. Curcumin and genistein, plant natural products, show synergistic inhibitory effect on the growth of human breast cancer MCF-7 cells induced by estrogenic pesticides. Biochem Biophys Res Commun 1997; 233: 692-6.
[25] Chanskaow S, Ishikawa T, Sekin K, Okada M, Higuchi Y, Kudo M, Chaichantipyuth C. Isoflavonoids from Pueraira mirifica and their estrogenic activity. Planta Med 2000; 66: 572-5.
[26] Fotsis T, Pepper M, Adlercreuts H, Flischmann G, Hase T. Genistein a dietary derived inhibitor of in vitro angiogenesis. Proc Natl Acad Sci USA 1993; 90: 2690-4.
[27] Gaudette DC, Holub BJ. Effect of genistein, a tyrosine kinase inhibitor, on U46619-induced phosphoinoside phosphorylation in human platelets. Biochem Biophys Res Commun 1990; 170: 238-42.
[28] Santen RJ. Inhibition of aromatase: insights from recent studies. Steroids 2003; 68: 559-67.
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