In Vivo Study of Diethylstilbestrol Teratogenicity on Mouse Embryo Teratogenicity of diethylstilbestrol
Iranian Journal of Pharmaceutical Sciences,
Vol. 1 No. 4 (2005),
1 October 2005
,
Page 237-241
https://doi.org/10.22037/ijps.v1.39545
Abstract
Diethylstilbestrol (DES) was widely used in the past, as the morning after contraception, but its application became extremely limited due to several complications including delayed clear cell adenocarcinoma in female infants. However, the use of DES increased during the past decade for hormone replacement therapy. The aim of this study was to investigate possible teratogenicity of this synthetic oestrogen. The experiment was conducted on N-MRI mice. Various concentrations of DES were administered i.p. to pregnant animals throughout the period of organogenesis (days 9 and 10 of pregnancy). The control group received ethanol as vehicle. Pregnancy was terminated on the 18th day by cervical dislocation. The embryos were then removed and fixed in Bouin’s solution, and parameters currently used in teratogenic studies were assessed. Severe embryo toxicity score was observed following the application of DES at doses of 200 and 400 mg/kg (71.4% and 83.6%, respectively). The weight and the average size of some of the examined parameters were markedly decreased by embryological observation. Furthermore, the size of derm and mosaic cells of urinary bladder, shortening in the length of femur, and abnormal disposition of calcium compound in embryos were markedly different in the treated mice.
- Diethylstylbestrol
- Mice embryo
- Teratogenicity
How to Cite
References
[2] Lynch HT, Reich JW. Diethylstilbestrol genetics, teratogenesis, and tumor spectrum in humans. Med Hypotheses 1985; 16: 315-32.
[3] Mittendorf R. Teratogen update: Carcinogenesis and teratogenesis associated with exposure to diethylstilbestrol in utero. Teratology 1995; 51: 35-45.
[4] Giusti RM, Iwam OK, Hatch EE. Diethylstilbestrol revisesd: a review of the long term health effects. Ann Intern Med 1995; 122: 778-88.
[5] Bibbo M, Gill W, Azizi F, Blough R, Fung VS, Rosenfield RL, Schumacher GF, Sleeper K, Sonek MG, Wied GL. Follow-up study of male and female offspring of diethylstilbestrol exposed mothers. Obstet Gynecol 1977; 49: 1-8.
[6] Rogers JM, Kavlock RJ. Developmental Toxicology. In: Klassen CD, (editor). Casarett and Doull’s toxicology, the basic science of poisons. New York: McGraw-Hill, 1996; pp. 301-31.
[7] Wardell RE, Seegmiller RE, Bradshaw WS. Induction of prenatal toxicity in the rat by diethylstilbestrol, zeranol, 3,4,3’,4’-tetrachlorobiphenyl, cadmium and lead. Teratology 1982; 26: 229-37.
[8] Beyer BK, Greenaway JC, Fantel AG, Juchau MR. Embryotoxicity induced by diethylstilbestrol in vitro. J Biochem Toxicol 1987; 2: 77-92.
[9] Gilabert J, Estelles A, Cano A, Espana F, Barrachnia R, Grancha S, Aznar J, Tortajada M. The effect of estrogen replacement therapy with or without progestogen on the fibrinolytic system and coagulation inhibitors in postmenopausal status. Am J Obstet Gynecol 1995; 173:1849-54.
[10] Perez-Martinez C, Garcia-Iglesias MJ, Bravo- Moral AM, Ferreras-Estrada MC, Martinez- Rodriguez JM, Escudero A. Effect of diethylstilbestrol or zeran on fetal development, gestation duration, and number of offspring in NMRI mice. Am J Vet Res 1995; 56: 1615-9.
- Abstract Viewed: 266 times
- IJPS_Volume 1_Issue 4_Pages 237-241 Downloaded: 184 times