Anti-Inflammatory, Analgesic Activities, and Phytochemical Study of Traganum nudatum Delile Biological effect and composition of Traganum nudatum Delile
Iranian Journal of Pharmaceutical Sciences,
Vol. 17 No. 3 (2021),
1 Tir 2021
,
Page 1-22
https://doi.org/10.22037/ijps.v17.40269
Abstract
Traganum nudatum Delile is a North African endemic medicinal plant commonly used in traditional medicine to treat wounds, hemorrhoids, rheumatism, and ear infections. Nevertheless, there are no scientific reports on the anti-inflammatory and analgesic effects of this plant. The present study aims to assess the therapeutic effect of the aqueous extract of the aerial part of the plant at three doses (40, 80, and 120 mg/kg) on experimentally pain-induced animals. First, the anti-inflammatory activity was assessed by carrageenan-induced paw edema on Swiss albino mice. Secondly, the analgesic activity was assessed by the acetic acid-induced writhing test. In addition, UPLC-MS-PDA and GC-MS-FID analyses were performed to screen the possible therapeutic compounds. The anti-inflammatory effect of the aqueous extract at 120 mg/kg for 4 h experiment was significantly higher (89.97± 0.17%) than those of the reference drug acetylsalicylic acid (ASA) (0± 0.25%);. In comparison, the analgesic test showed a remarkable reducing pain effect (69.8 ± 1.7%) at the dose of 80 mg/kg, almost similar to those of Acetaminophen (Paracetamol) (72.54 ± 2.26 %). The phytochemical screening revealed the presence of therapeutic biomolecules such as flavonoids, mainly rutin (6440± 3.0 μg/g), flavonols (narcissoside 115.1± 1.4 μg/g) and phenolic acids (chlorogenic acid 1480± 1.6 μg/g). GC-MS-FID showed the presence of saturated acids such as behenic and palmitic acid with percentages of 35.58 ± 0.06 % and 17.54 ± 0.09 %, respectively. These were in a higher percentage than the unsaturated fatty acids. These results validated the use of T.nudatum Delile for treating inflammatory and analgesic disorders in folk medicine. The presence of bioactive compounds, including polyphenols and fatty acids, may explain the pharmacological effect of the medicinal plant.
- Analgesic
- Anti-inflammatory
- GC-MS-FID
- Polyphenols
- Traganum nudatum Delile
- UPLC-MS-PDA
How to Cite
References
[2] Bellakhdar J. Plantes médicinales au Maghreb et soins de base- Précis de phytothérapie moderne, Le fennec. Casablanca (2006).
[3] Duraffourd C and Lapraz JC CR. La plante médicinale de la tradition à la science. Jacques Grancher publishers, Paris (1997).
[4] Telli A, Esnault M A and Ould El Hadj Khelil A. An ethnopharmacological survey of plants used in traditional diabetes treatment in south-eastern Algeria (Ouargla province). J. Arid Environ. (2016) 127: 82–92.
[5] Mouderas F, El Haci IA, and Lahfa FB. Phytochemical profile, antioxidant and antimicrobial activities of Traganum nudatum Delile aerial parts organic extracts collected from Algerian Sahara’s flora. Orient. Pharm. Exp. Med (2019) 19: 299–310.
[6] Winter CA, Risley EA and Nuss GW. Carrageenin-Induced Edema in Hind Paw of the Rat as an Assay for Anti-inflammatory Drugs. Exp. Biol. Med. (1962) 111: 544–547.
[7] Koster R, Anderson M and De Beer E. Acetic acid for analgesic screening. Fed. Proc. (1959) 18: 412–430.
[8] Collier HO, Dinneen LC, Johnson CA and Schneider C. The abdominal constriction response and its suppression by analgesic drugs in the mouse. Br. J. Pharmacol. Chemother. (1968) 32: 295–310.
[9] Sawadogo WR, Boly R, Lompo M, Some N, Lamien CE, Guissou IP and Nacoulma OG. Anti-inflammatory, analgesic and antipyretic activities of dicliptera verticillata. Int. J. Pharmacol. (2006) 2 (4): 435–438.
[10] Das B, Ferdous T, Mahmoud Q, Hannan JMA, Bhattacharjee R and Das BK. Antinociceptive and Anti-inflammatory Activity of the Bark Extract of Plumeria rubra on Laboratory Animals. European Med. Plants. (2013) 3 (1): 114–126.
[11] Delpino-Rius A, Eras J, Vilaró F, Cubero MÁ, Balcells M and Canela-Garayoa R. Characterization of phenolic compounds in processed fibers from the juice industry. Food Chem. (2015) 172: 575–584.
[12] Su D, Zhang R, Hou F, Zhang M, Guo J, Huang F,Deng Y and Wei Z. Comparison of the free and bound phenolic profiles and cellular antioxidant activities of litchi pulp extracts from different solvents. BMC. Complement. Altern. Med. (2014) 14: 9.
[13] Nardini M, Cirillo E, Natella F, Mencarelli D, Comisso A and Scaccini C. Detection of bound phenolic acids: prevention by ascorbic acid and ethylenediaminetetraacetic acid of degradation of phenolic acids during alkaline hydrolysis. Food Chem. (2002) 79 (1): 119–124.
[14] Woodring PJ, Edwards PA and Chisholm MG. HPLC determination of non-flavonoid phenols in vidal blanc wine using electrochemical detection. J. Agric. Food Chem. (1990) 38: 729–732.
[15] Porter LJ. Number- and Weight-Average Molecular Weights for Some Proanthocyanidin Polymers (Condensed Tannins). Aust. J. Chem. (1986) 39 (4): 557–562.
[16] Eras J, Ferran J, Perpiña B and Canela R. Chlorotrimethylsilane, a reagent for the direct quantitative analysis of fats and oils present in vegetable and meat samples. J. Chromatogr. A. (2004) 1047 (1): 157–161.
[17] Balamurugan K, Nishanthini A and Mohan VR. GC–MS analysis of Polycarpaea corymbosa (L.) Lam whole plant. Asian Pac. J. Trop. Biomed. (2012) 2 (3): 1289–1292.
[18] Brooks PM and Day RO. Nonsteroidal Anti-inflammatory- Drugs Differences and Similarities. N. Engl. J.Med .(1991) 324 (24): 1716–1725.
[19] Morris JL, Rosen DA and Rosen KR. Nonsteroidal anti-inflammatory agents in neonates. Pediatric Drugs. (2003) 5: 385–405.
[20] Ajayi AM, Tanayen JK, Magomere A and Ezeonwumelu JOC. Antinociceptive and anti-inflammatory effects of aqueous extract of Chenopodium opulifolium schrad leaves. J. Intercult. Ethnopharmacol. (2017) 6 (1): 14–21.
[21] Soro TY, Traore F and Sakande J. Activité analgésique de l’extrait aqueux de Ximenia americana (Linné) (Olacaceae). Comptes Rendus. Biol. (2009) 332 (4): 371–377.
[22] Hasan SMR, Jamila M, Majumder MM, Akter R, Hossain MM, Mazumder MDEH, Alam MA, Jahangir R, Md. Rana MS, Arif M and Rahman S. Analgesic and antioxidant activity of the hydromethanolic extract of Mikania scandens (L.) Willd. leaves. Am. J. Pharmacol. Toxicol. (2009) 4 (1): 1–7.
[23] Ferreira SH, Moncada S and Vane JR. Prostaglandins and the mechanism of analgesia produced by aspirin-like drugs. Br. J. Pharmacol. (1997) 120 (1): 401–412.
[24] Ibironke GF and Ajiboye KI. Studies on the anti-inflammatory and analgesic properties of Chenopodium ambrosioides leaf extract in rats. Int. J. Pharmacol. (2007) 3: 111–115.
[25] Repo CVR, Hellström JK, Pihlava JM and Mattila PH. Flavonoids and other phenolic compounds in Andean indigenous grains: Quinoa (Chenopodium quinoa), kañiwa (Chenopodium pallidicaule) and kiwicha (Amaranthus caudatus). Food Chem. (2010) 120 (1): 128–133.
[26] Kim TH, Ku S-K and Bae J-S. Anti-inflammatory activities of isorhamnetin-3-O-galactoside against HMGB1-induced inflammatory responses in both HUVECs and CLP-induced septic mice. J. Cell. Biochem. (2013) 114 (2): 336–345.
[27] Brimson JM, Onlamoon N, Tencomnao T and Thitilertdecha P. Clerodendrum petasites S. Moore: The therapeutic potential of phytochemicals, hispidulin, vanillic acid, verbascoside, and apigenin. Biomed. Pharmacother. (2019) 118: 109319.
[28] Ganeshpurkar A and Saluja AK. The Pharmacological Potential of Rutin. Saudi Pharm. J. (2017) 25 (2): 149–164.
[29] Lin LC, Pai YF and Tsai TH. Isolation of Luteolin and Luteolin-7-O-glucoside from Dendranthema morifolium Ramat Tzvel and Their Pharmacokinetics in Rats. J. Agric. Food Chem. (2015) 63 (35): 7700–7706.
[30] Majouli K, Hamdi A, Msaada K and Kenani A. A bioactivity guided study on the antibacterial activity of Hertia cheirifolia L. extracts. Microb. Pathog. (2017) 106: 113–118.
[31] Touil YS, Auzeil N, Boulinguez F, Saighi H, Regazzetti A, Scherman D, Chabot GG. Fisetin disposition and metabolism in mice: Identification of geraldol as an active metabolite. Biochem.Pharmacol. (2011) 82 (11): 1731–1739.
[32] Matsumoto H, Inaba H, Kishi M, Tominaga S, Hirayama M and Tsuda T. Orally Administered Delphinidin 3-Rutinoside and Cyanidin 3-Rutinoside Are Directly Absorbed in Rats and Humans and Appear in the Blood as the Intact Forms. J. Agric. Food Chem. (2001) 49 (3): 1546–1551.
[33] Jesus RS, Piana M, Freitas RB, Brum TF, Alves CFS, Belke B V, Mossmann NJ, Cruz RC, Santos RCV, Dalmolin TV, Bianchini BV, Campos MAA and De Freitas Bauermann L. In vitro antimicrobial and antimycobacterial activity and HPLC–DAD screening of phenolics from Chenopodium ambrosioides L. Brazilian J. Microbiol. (2018) 49 (2): 296–302.
[34] Naveed M, Hejazi V, Abbas M, Kamboh AA, Khan GJ, Shumzaid M, Ahmad F, Babazadeh D, FangFang X, Modarresi-Ghazani F, WenHua L and XiaoHui Z. Chlorogenic acid (CGA): A pharmacological review and call for further research. Biomed. Pharmacother. (2018) 97: 67–74.
[35] Bittrich V and Amaral MDCE. Proanthocyanidins in the testa of centrospermous seeds. Biochem. Syst. Ecol. (1991) 19 (4): 319–321.
[36] Nisar M, Shah H, Khan I, Fazal-ur-Rehman, Khan MS, Marwat SK, Niazi ZR and Ullah A. Antimicrobial Potential and Phytochemical Investigation of Fixed Oil of Plant Chenopodium ambrosioides Linn. Asian J. Chem. (2013) 25 (2): 1069–1072.
[37] Mullen A, Loscher CE and Roche HM. Anti-inflammatory effects of EPA and DHA are dependent upon time and dose-response elements associated with LPS stimulation in THP-1-derived macrophages. J.
Nutr. Biochem. (2010) 21 (5): 444–450.
[38] Pereira LM, Hatanaka E, Martins EF, Oliveira F,Liberti EA, Farsky SH, Curi R and Pithon-Curi TC. Effect of oleic and linoleic acids on the inflammatory phase of wound healing in rats. Cell Biochem. Funct. (2008) 26 (2): 197–204
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