Potency of Mangosteen Pericarp Extract to Inhibit 38-kDa and Ag85 Protein Secretion by Mycobacterium tuberculosis H37Rv Mangosteen pericarp inhibit Tuberculosis Protein secretion
Iranian Journal of Pharmaceutical Sciences,
Vol. 16 No. 2 (2020),
1 April 2020
,
Page 19-26
https://doi.org/10.22037/ijps.v16.40421
Abstract
Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. The α-mangostin in mangosteen pericarp extract can inhibit M. tuberculosis growth. This study examined the potency of α-mangostin in mangosteen pericarp extract to inhibit 38-kDa and Ag85 protein secretion from M. tuberculosis H37Rv. The samples used in this study were divided into three independent variable groups (3.125 μg/ml; 6.25 μg/ml; and 12.5 μg/ml α-mangostin in the mangosteen pericarp extract), a positive control group (rifampin), a negative control group (M. tuberculosis H37Rv), and the Garcia® group (trademark of the mangosteen peel extract capsule). HPLC-MS/MS detected that the mangosteen pericarp extract contained 5,984.55 μg/g α-
mangostin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and dot blot analysis were conducted to analyze the profile and specificity of both proteins. This study revealed that 6.25 μg/ml of mangosteen pericarp extract had the greatest potency for inhibiting 38-kDa protein secretion from M. tuberculosis H37Rv, and 12.5 μg/ml mangosteen pericarp extract had greater potency for inhibiting Ag85 protein secretion than did the other doses. Thus, α-mangosteen from mangosteen pericarp extract can inhibit Ag38 and Ag85 secretion.
- α-mangostin
- Ag38 kDa
- Ag85
- Mycobacterium tuberculosis
How to Cite
References
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