Evaluation of Ibuprofen Release from Gelatin /Hydroxyapatite /Polylactic Acid Nanocomposites Ibuprofen Release from Gelatin /Hydroxyapatite /Polylactic Acid Nanocomposites
Iranian Journal of Pharmaceutical Sciences,
Vol. 14 No. 1 (2018),
15 January 2018
,
Page 75-84
https://doi.org/10.22037/ijps.v14.40674
Abstract
Gelatin-hydroxyapatite-polylactic acid (PLA) nanocomposites were synthesized using five different formulations. The nanocomposites were loaded with ibuprofen and the amount of drug in the carriers was determined. X-ray diffraction (XRD) analysis was conducted before and after drug loading to ensure the presence of ibuprofen on the nanocomposites. Drug delivery was evaluated in phosphate buffered saline (PBS) solution at pH 7.4 and 37°C. The results of XRD analysis showed acceptable synthesis of hydroxyapatite and the composites, confirming the loading of ibuprofen onto the synthesized nanocarriers. The results showed that maximum drug loading (58.2%) was recorded for sample D (30% gelatin, 40% nHA and 30% PLA), and minimum loading was recorded for sample E (30% gelatin, 30% nHA and 40% PLA). The maximum percentage of drug release over the course of 72 h (95.8%) was for nanocomposite D (30% gelatin, 40% nHA and 30% PLA). The minimum percentage of drug delivery (77%) was for nanocomposite E (30% gelatin, 30% nHA, 40% PLA), which contained the maximum PLA content.
Keywords:
- Nanocomposites
- Hydroxyapatite
- Polylactic acid
- Ibuprofen
- Release profile
- Drug delivery
How to Cite
Zohre Nabipour, Mohammad Sadegh Nourbakhsh, & Mohammad Baniasadi. (2018). Evaluation of Ibuprofen Release from Gelatin /Hydroxyapatite /Polylactic Acid Nanocomposites: Ibuprofen Release from Gelatin /Hydroxyapatite /Polylactic Acid Nanocomposites. Iranian Journal of Pharmaceutical Sciences, 14(1), 75–84. https://doi.org/10.22037/ijps.v14.40674
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[2] Whitman W. Drug Administration and Drug Effectiveness. Saltzman WM, (Eds.) In: Drug Delivery: Engineering Principles for Drug Therapy, New York: Oxford University Press (2001) 8–19.
[3] Verron E, Khairoun I, Guicheux J, Bouler JM. Calcium phosphate biomaterials as bone drug delivery systems: a review. Drug discovery today (2010)15(13): 547-52.
[4] Bahrololoom ME, Javidi M, Javadpour S, Ma J. Characterisation of natural hydroxyapatite extracted from bovine cortical bone ash. J Ceram. Process. Res. (2009)10: 129-38.
[5] Kamalanathan P, Ramesh S, Bang LT, Niakan A, Tan CY, Purbolaksono J, Chandran H, Teng, WD, Synthesis and sintering of hydroxyapatite derived from eggshells as a calcium precursor. Ceramics International (2014)40(10): 16349-59.
[6] Liu TY, Chen SY, Liu DM, Liou SC. On the study of BSA-loaded calcium-deficient hydroxyapatite nano-carriers for controlled drug delivery. J of Controlled Release (2005)107(1): 112-21.
[7] Lian X, Mao K, Liu X, Wang X, Cui F. In vivo osteogenesis of vancomycin loaded nanohydroxyapatite/collagen/calcium sulfate composite for treating infectious bone defect induced by chronic osteomyelitis. J of Nanomaterials (2015)2015: 13.
[8] Palazzo B, Sidoti MC, Roveri N, Tampieri A, Sandri M, Bertolazzi L, Galbusera F, Dubini G, Vena P, Contro R. Controlled drug delivery from porous hydroxyapatite grafts: An experimental and theoretical approach. Materials Science and Engineering: C (2005)25: 207-13.
[9] Liu WC, Wong CT, Fong MK, Cheung WS, Kao RY, Luk KD, Lu WW. Gentamicin‐loaded strontium‐containing hydroxyapatite bioactive bone cement An efficient bioactive antibiotic drug delivery system. J of Biomedical Materials Research Part B: Applied Biomaterials (2010)95(2): 397-406.
[10] Joosten U, Joist A, Frebel T, Brandt B, Diederichs S, Von Eiff C. Evaluation of an in situ setting injectable calcium phosphate as a new carrier material for gentamicin in the treatment of chronic osteomyelitis: studies in vitro and in vivo. Biomaterials (2004)25: 4287-95.
[11] Haghbin-Nazarpak MA, Moztarzadeh F, Solati-Hashjin ME, Mirhabibi AR, Tahriri MO. Preparation, characterization and Gentamicin sulfate release investigation of biphasic injectable calcium phosphate bone cement. Ceram-Silik (2010)54: 334-40.
[12] Vincent JFV. Structural biomaterials, 1st ed. Princeton University Press: USA (2012).
[13] Fang Z, Feng Q, Tan R. In-situ grown hydroxyapatite whiskers reinforced porous HA bioceramic. Ceramics International (2013)39(8): 8847-52.
[14] Koo OM, Rubinstein I, Onyuksel H. Role of nanotechnology in targeted drug delivery and imaging: a concise review. Nanomedicine: Nanotechnology, Biology and Medicine (2005)1: 193-212.
[15] Jones RAL. Soft Mashines: Nanotechnology and Life, 1st ed. Oxford University Press: New York (2008).
[16] Kim Y, White JL. Melt‐intercalation nanocomposites with fluorinated polymers and a correlation for nanocomposite formation. J of applied polymer science (2004)92: 1061-71.
[17] Kim H, Che L, Ha Y, Ryu W. Mechanically-reinforced electrospun composite silk fibroin nanofibers containing hydroxyapatite nanoparticles. Materials Science and Engineering: C (2014)40: 324-35.
[18] Becker J, Lu L, Runge MB, Zeng H, Yaszemski MJ, Dadsetan M. Nanocomposite bone scaffolds based on biodegradable polymers and hydroxyapatite. J of Biomedical Materials Research Part A (2015)103(8): 2549-57.
[19] Mishra B, Patel BB, Tiwari S. Colloidal nanocarriers: a review on formulation technology, types and applications toward targeted drug delivery. Nanomedicine: Nanotechnology, biology and medicine (2010)6: 9-24.
[20] Danhier F, Feron O, Préat V. To exploit the tumor microenvironment: passive and active tumor targeting of nanocarriers for anti-cancer drug delivery. J of Controlled Release (2010)148: 135-46.
[21] Ramot Y, Haim-Zada M, Domb AJ, Nyska A. Biocompatibility and safety of PLA and its copolymers. Advanced drug delivery reviews (2016)107: 153-62
[22] Chen L, Ma L, Zhou M, Liu Y, Zhang Y. Effects of pressure on gelatinization of collagen and properties of extracted gelatins. Food Hydrocolloids (2014)36: 316-22.
[23] Alizadeh M, Abbasi F, Khoshfetrat AB, Ghaleh H. Microstructure and characteristic properties of gelatin/chitosan scaffold prepared by a combined freeze-drying/leaching method. Materials Science and Engineering: C (2013)33(7): 3958-67.
[24] Wisotzki EI, Hennes M, Schuldt C, Engert F, Knolle W, Decker U, Käs JA, Zink M, Mayr SG. Tailoring the material properties of gelatin hydrogels by high energy electron irradiation. J of Materials Chemistry B (2014)2(27): 4297-309.
[25] Eraga SO, Iwuagwu MA. Formulation and evaluation of bioadhesive tablets of Metronidazole from Gellan gum and gelatin. J of Pharmacy Research (2014)8(8): 1132-9.
[26] Notodihardjo PV, Morimoto N, Kakudo N, Matsui M, Sakamoto M, Liem PH, Suzuki K, Tabata Y, Kusumoto K. Gelatin hydrogel impregnated with platelet-rich plasma releasate promotes angiogenesis and wound healing in murine model. J of Artificial Organs. (2015)18(1): 64-71.
[27] Tanaka K, Shiga T, Katayama T. Fabrication Of Hydroxyapatite/PLA Composite Nanofiber By Electrospinning. WIT Transactions on The Built Environment (2016)166: 371-9
[28] Samadikuchaksaraei A, Gholipourmalekabadi M, Erfani Ezadyar E, Azami M, Mozafari M, Johari B, Kargozar S, Jameie SB, Korourian A, Seifalian AM. Fabrication and in vivo evaluation of an osteoblast‐conditioned nano‐hydroxyapatite/gelatin composite scaffold for bone tissue regeneration. of Biomedical Materials Research Part A (2016)104 (8): 2001-10
[29] Nabipour Z, Nourbakhsh MS, Baniasadi M. Synthesis, characterization and biocompatibility evaluation of hydroxyapatite-gelatin polyLactic acid ternary nanocomposite. Nanomedicine Journal (2016)3: 127-34.
[30] Patel S, Wei S, Han J, Gao W. Transmission electron microscopy analysis of hydroxyapatite nanocrystals from cattle bones. Materials Characterization (2015)109: 73-8.
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