Evaluation of Neuroprotective Effect of Althaea Officinalis Flower Aqueous and Methanolic Extracts against H2O2-Induced Oxidative Stress in PC12 Cells Protective activity of Althaea Officinalis against oxidative stress
Iranian Journal of Pharmaceutical Sciences,
Vol. 13 No. 1 (2017),
1 January 2017
,
Page 49-56
https://doi.org/10.22037/ijps.v13.40705
Abstract
This study was conducted to evaluate the possible antioxidant activity and neuroprotective effects of aqueous and methanolic extracts of Althaeaofficinalis flowers against H2O2-induced oxidative stress in PC12 cells.
The antioxidant potential of extracts was evaluated by radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. For cytoprotective activity, the cells were pretreated with different concentrations (12.5, 25, 50, 100, 200and 400 μg/ml) of the extracts for 24h and then incubated with H2O2(480 μM) for 3 h. In co-treatment protocol, cells were simultaneously treated with H2O2 (480 μM) and the same concentrations of extracts, used in pretreatment protocol. Percentage of viability was measured using MTT assay. The aqueous and methanolic extracts did not show strong DPPH radical scavenging activity (IC50 value of 128 and 255 μg/ml respectively) in comparison with ascorbic acid (IC50 value of 6.1 μg/ml). The cytoprtection study revealed that neither the methanolic, northe aqueous extractsat tested concentrations could protect the cells against H2O2-induced cytotoxicity compared to H2O2 alone, in either co-treatment or pre-treatment experiments. Despite reporting the antioxidant activity of A. officinalis L. flowers, it seems that such a negligible cytoprotective activity may be related to some other factors. On the other hand, the presence of moderate antioxidant activity does not guarantee the protective activity against oxidative stress.
- Althaeaofficinalis
- Neuroprotective
- DPPH assay
- Oxidative stress
- PC12 cell line
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References
[2]Poljsak, B., D. Šuput and I. Milisav (2013). Achieving the balance between ROS and antioxidants: when to use the synthetic antioxidants. Oxid Med Cell Longev 2013.
[3] Uttara, B., A. V. Singh, P. Zamboni and R. Mahajan (2009). Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol 7, 65-74.
[4] Burgener, S., L. Buettner, K. C. Buckwalter, E. Beattie, A. Bossen, D. Fick, S. Fitzsimmons, A. Kolanowski, N. Richeson and K. Rose (2008). Evidence supporting nutritional interventions for persons in early stage Alzheimer’s disease (AD). J Nutr Health Aging12, 18-21.
[5] M. Blumenthal, A. G., J. Brinckmann (Eds.), (2000). Herbal Medicine: Expanded Commission E Monographs, Integrative Medicine Communications, Boston.
[6] Šutovská, M., G. Nosáľová, J. Šutovský, S. Fraňová, L. Prisenžňáková and P. Capek (2009). Possible mechanisms of dose-dependent cough suppressive effect of Althaea officinalis rhamnogalacturonan in guinea pigs test system. Int. J. Biol. Macromolec.45(1): 27-32.
[7] Deters, A., J. Zippel, N. Hellenbrand, D. Pappai, C. Possemeyer and A. Hensel (2010). Aqueous extracts and polysaccharides from Marshmallow roots (Althea officinalis L.): Cellular internalisation and stimulation of cell physiology of human epithelial cells in vitro J ethnopharmacol 127, 62-69.
[8] Kardošová A, Machová E. Antioxidant activity of medicinal plant polysaccharides. Fitoterapia. 2006;77(5):367-73.
[9] Iauk L, Lo Bue A, Milazzo I, Rapisarda A, Blandino G. Antibacterial activity of medicinal plant extracts against periodontopathic bacteria. Phytother Res. 2003;17(6):599-604.
[10] Hage-Sleiman R, Mroueh M, Daher CF. Pharmacological evaluation of aqueous extract of Althaea officinalis flower grown in Lebanon. Pharm. Biol. 2011;49(3):327-33.
[11]Rezaei M, Alirezaei M. Protective effects of Althaea officinalis L. extract in 6-hydroxydopamine-induced hemi-Parkinsonism model: behavioral, biochemical and histochemical evidence. J Physiol Sci. 2014;64(3):171-6.
[12] Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Pnas. 1976;73(7):2424-8.
[13] Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature 181, 1199 - 1200.
[14] Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1-2):55-63
[15] Matés JM, Sánchez-Jiménez FM. Role of reactive oxygen species in apoptosis: implications for cancer therapy. Int. J. Biochem. Cell Biol. 2000;32(2):157-70.
[16] Shah S, Akhtar N, Akram M, Shah PA, Saeed T, Ahmed K, et al. Pharmacological activity of Althaea officinalis L. J Med Plants Res. 2011;5:5662-6.
[17] Elmastas M, Ozturk L, Gokce I, Erenler R, Aboul‐Enein HY. Determination of antioxidant activity of marshmallow flower (Althaea officinalis L.). Anal let. 2004;37(9):1859-69.
[18] Mishra K, Ojha H, Chaudhury NK. Estimation of antiradical properties of antioxidants using DPPH assay: A critical review and results. Food Chemistry. 2012;130(4):1036-43.
[19] Sadighara P, Gharibi S, Jafari AM, Khaniki GJ, Salari S. The antioxidant and Flavonoids contents of Althaea officinalis L. flowers based on their color. AJP. 2012;2(3):113.
[20] Saha K, Lajis N, Israf D, Hamzah A, Khozirah S, Khamis S, et al. Evaluation of antioxidant and nitric oxide inhibitory activities of selected Malaysian medicinal plants. J Ethnopharmacol. 2004;92(2):263-7.
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