Assessment of Heavy Metal Accumulation and Antibacterial Activity of Some Medicinal Herbs Grown in an Industrial Area of Steel Production, Ahvaz Assessment of Heavy Metal Accumulation and Antibacterial Activity
Iranian Journal of Pharmaceutical Sciences,
Vol. 13 No. 1 (2017),
1 January 2017
,
Page 73-86
https://doi.org/10.22037/ijps.v13.40707
Abstract
Unfavorable conditions can have effect on secondary metabolites production in the plants. The aim of present study was to evaluate the effect of heavy metals accumulation on antibacterial potential of Chenopodium murale L., Senecio glaucus L., Malva parviflora L. and Calendula arvensis L. grown in an industrial area of steel production in Ahvaz, southwest of Iran. The cadmium (Cd), iron (Fe), manganese (Mn), nickel (Ni), lead (Pb) and zinc (Zn) values were analyzed in different parts of these medicinal herbs. The antibacterial activity of methanol and ethanol extracts of the plants was surveyed in standard disc diffusion method against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa. In addition, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of these extracts were determined. High concentration of Cd was found in the roots of M. parviflora and S. glaucus. The concentration of other metals in these plants was within the allowed limits. Both of ethanolic and metanolic extracts exhibited the highest antibacterial effect against E. coli and P. aeruginosa. MIC and MBC values of alcoholic extracts were determined against E. coli and P. aeruginosa as the most sensitive bacterial species. It seems that antibacterial activity of studied plants is not essentially correlated with their heavy metal concentration.
Keywords:
- Alcohol extracts
- Antibacterial agents
- Ethanolic extracts
- Medicinal plants
- Metals assay
- Methanolic extracts
How to Cite
Parzhak Zoufan, Roya Jalali, Amer Karimiafshar, & Hossein Motamedi. (2017). Assessment of Heavy Metal Accumulation and Antibacterial Activity of Some Medicinal Herbs Grown in an Industrial Area of Steel Production, Ahvaz: Assessment of Heavy Metal Accumulation and Antibacterial Activity. Iranian Journal of Pharmaceutical Sciences, 13(1), 73–86. https://doi.org/10.22037/ijps.v13.40707
References
[1] Fritsch et al. Spatial distribution of metals in smelter impacted soils of woody habitats: influence of landscape and soil properties and risk for wildlife. Chemosphere (2010) 81(2): 141-155.
[2] Nouri J, Khorasani, N, Lorestani B, Karami M, Hassani N, Yousefi N. Accumulation of heavy metals in soil and uptake by plant species with phytoremediation potential. Environ Earth Sci (2009) 59 (2): 315-323.
[3] Ataabadi M, Hoodaji M, Najafi P. Heavy metals biomonitoring by plants grownin an industrial area of Isfahan' Mobarakeh steel company. J Environ Stud (2010) 35(52): 25-27.
[4] Ghaderian SM, Ghotbi Ravandi AA. Accumulation of copper and other heavy metals by plants growing on Sarcheshmeh copper mining area, Iran. J Geochem Explor (2012) 123: 25-32.
[5] Zoufan P, Saadatkhah A, Rastegarzadeh S. Amount of Mn and Zn in herbaceous plants growing on industrial area of steel production companies in southeast of Ahvaz, Iran. Prog Biol Sci (2015) 5 (2): 181-193.
[6] Dwivedi SK, Dey S. Medicinal herbs: A potential source of toxic metal exposure for man and animals in India. Arch Environ Health (2002) 57 (3): 229-231.
[7] Steenkamp V, Stewart MJ, Curowska E, Zuckerman M. A severe case of multiple metals poisoning in a child treated with a traditional medicine. Forensic Sci Int (2002)128(3): 123-126.
[8] World Health Organization: WHO Guidelines for assessing quality of herbal medicines with reference to contaminants and residues. WHO Publishers: Geneva (2007).
[9] Ajasa A, Bello MO, Ibrahim AO, Ogunwande IA, Olawore NO. Heavy trace metals and macronutrients status in herbal plants of Nigeria. Food Chem (2004) 85 (1): 67-71.
[10] Desideri D, Meli MA, Roselli C. Determination of essential and non-essential elements in some medicinal plants by polarized X ray fluorescence spectrometer (EDPXRF). Microchem J (2010) 95 (2): 174-180.
[11] Gajalakshmi S, Iswarya V, Ashwini R, Divya G, Mythili S, Sathiavelu A. Evaluation of heavy metals in medicinal plants growing in Vellore District. Eur J Exp Biol (2012) 2 (5): 1457-1461.
[12] Debnath M, Khandelwal M, Lal P, Jain R. Evaluation of heavy metal distribution and antibacterial activities of medicinal plants Tinospora cordifolia, Ocimum sanctum and Piper nigrum. Int J Pharm Sci Drug Res (2014) 6 (3): 229-234.
[13] Okem A, Southway C, Stirk WA, Street RA, Finnie JF, Van Staden J. Heavy metal contamination in South African medicinal plants: a cause for concern. S Afr J Bot (2014) 93: 125-130.
[14] Salehi P, Asghari B, Mohammadi F. Biosorption of Ni(II), Cu(II) and Pb(II) by Punica granatum from aqueous solutions. J Water Resour Prot (2010) 2 (8): 701-705.
[15] Street RA, Kulkarni MG, Stirk WA. Southway C, Van Staden J. Variation in heavy metals and microelements in South African medicinal plants obtained from street markets. Food Addit Contam (2008) 25 (8): 953-960.
[16] Khan H, Khan MA, Mahmood T, Choudhary MI. Antimicrobial activity of Gloriosa superba L. (Colchicaceas) extracts. J Enzyme Inhib Med Chem (2008) 23 (6): 855-859.
[17] Rascio N, Navari-Izzo F. Heavy metal hyperaccumulating plants: How and why do they do it? And what makes them so interesting? Plant Sci (2011) 180(2): 169-181.
[18] Okem A, Southway C, Stirk WA, Street RA, Finnie JF, Van Staden J. Effect of cadmium and aluminum on growth, metabolite content and biological activity in Drimia elata (Jacq.) Hyacinthaceae. S Afr J Bot (2015) 98: 142-147.
[19] Akinpelu DA, Aiyegoro OA, Okoh AI. The in vitro antioxidant property of methanolic extract of Afzelia africana (Smith.). J Med Plants Res (2010) 4 (19): 2021-2027.
[20] Kokanova-Nedialkova Z, Nedialkov PT, Nikolov SD. The genus Chenopodium: Phytochemistry ethnopharmacology and pharmacology. Pharmacogn Rev (2009) 3 (6): 280-306.
[21] El-Amier YA, Abdelghany AM, Abed Zaid A. Green synthesis and antimicrobial activity of Senecio glaucus - mediated silver nanoparticles. Res J Pharm Biol Chem Sci (2014) 5 (5): 631-642.
[22] Oladipupo LA, Adebola OO. Chemical composition of the essential oils of the flowers, leaves and stems of two Senecio polyanthemoides Sch. Bip. samples from South Africa. Molecules (2009) 14 (6): 2077-2086.
[23] Gasparetto JC, Martins CAF, Hayashi SS, Otuky MF, Pontarolo R. Ethnobotanical and scientific aspects of Malva sylvestris L.: a millennial herbal medicine. J Pharm Pharmacol (2011) 64 (2):172-189.
[24] Samavati V, Manoochehrizade A. Polysaccharide extraction from Malva sylvestris and its anti-oxidant activity. Int J Bio Macromol (2013) 60: 427-436.
[25] Jimenez-Arellanes A, Meckes M, Ramirez R, Torres J, Luna-Herrera J. Activity against multidrug-resistant Mycobacterium tuberculosis in Mexican plants used to treat respiratory diseases. Phytother Res (2013) 17 (8): 903-908.
[26] Paolini J, Barboni T, Desjobert JM, Djabou N, Muselli A, Costa J. Chemical composition, interspecies variation and seasonal variation in essential oils of Calendula arvensis L. Biochem Syst Ecol (2010) 38 (5): 865-874.
[27] Asadi-Samani M, Kafash-Farkhad N, Azimi N, Fasihi A, Alinia-Ahandani E, Rafieian-Kopaei M. Medicinal plants with hepatoprotective activity in Iranian folk medicine. Asian Pac J Trop Biomed (2015) 5 (2): 146-157.
[28] Erctin T, Senol FS, Orhan IE, Toker G. Comparative assessment of antioxidant and cholinesterase inhibitory properties of the marigold extracts from Calendula arvensis L. and Calendula officinalis L. Ind Crop Prod (2012) 36 (1): 203-208.
[29] Motamedi H, Seyyednejad SM, Hasannejad Z, Dehghani F. Comparative study on the effects of Ziziphus Spina-christi alcoholic extracts on growth and structural integrity of bacterial pathogens. Iran J Pharm Sci (2014)10 (2): 1-10.
[30] Kovacs B, Gyori Z, Prokisch J, Loch J, Daniel, P. A study of plant sample preparation and Inductively Coupled Plasma Emission Spectrometry parameters. Commun Soil Sci Plan (1996) 27 (5-8): 1177-1198.
[31] Soon YK, Abboud S. Cadmium, Chromium, Lead and Nickel. Carter MR (Ed.) In: Soil sampling and methods of analysis, Lewis Publishers CRC, Boca Raton (1993) 101-109.
[32] Hussain Z, Mohammed Khan K, Perveen S, Ambreeen N, Ur Rahman W, Ullah A. The effect of cadmium and chromium concentration, on biological activity of Marsilea minuta. J Chem Soc Pak (2011) 33 (6): 874-876.
[33] Hansch R, Mendel RR. Physiological function of mineral micronutrients (Cu, Zn, Mn, Fe, Ni, Mo, B, Cl). Curr Opin Plant Biol (2009)12 (3): 259-266.
[34] Choy CMY, Lam CW, Cheung LT, Briton-Jones CM, Cheung LP, Haines CJ. Infertility, blood mercury concentrations and dietary seafood consumption: A case-control study. Brit J Obstet Gynaec (2002) 109 (10): 1121-1125.
[35] Street RA, Kulkarni MG, Stirk WA, Southway C, Abdillahi HS, Chinsamy M, Van Staden J. Effect of cadmium uptake and accumulation on growth and antibacterial activity of Merwilla pumbea - an extensively used medicinal plant in South Africa. S Afr J Bot (2009) 75: 611–616.
[36] Noori M, Malayeri B, Moosaei M, Pakzad R, Piriye MH. Effects of heavy metals on the antibacterial properties of Verbascum speciosum Schard. Revista Cientifica UDO. Agrícola (2012) 12 (2): 463–471.
[37] Dorman HJD, Deans SG. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol (2000) 88: 308-316.
[38] Michalak A. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Pol J Environ Stud (2006)15 (4): 523-530.
[39] Marquez-Garcia B, Fernandez-Recamales MA, Cordoba F. Effects of cadmium on phenolic composition and antioxidant activities of Erica andevalensis. J Bot (2012) 2012: 1-6.
[40] Maksimovic ZA, Mravoic M, Dordevic S, Mraovic M. Antimicrobial activity of Chenopodium botrys essential oil. Fitoterapia (2005)76 (1): 112-114.
[2] Nouri J, Khorasani, N, Lorestani B, Karami M, Hassani N, Yousefi N. Accumulation of heavy metals in soil and uptake by plant species with phytoremediation potential. Environ Earth Sci (2009) 59 (2): 315-323.
[3] Ataabadi M, Hoodaji M, Najafi P. Heavy metals biomonitoring by plants grownin an industrial area of Isfahan' Mobarakeh steel company. J Environ Stud (2010) 35(52): 25-27.
[4] Ghaderian SM, Ghotbi Ravandi AA. Accumulation of copper and other heavy metals by plants growing on Sarcheshmeh copper mining area, Iran. J Geochem Explor (2012) 123: 25-32.
[5] Zoufan P, Saadatkhah A, Rastegarzadeh S. Amount of Mn and Zn in herbaceous plants growing on industrial area of steel production companies in southeast of Ahvaz, Iran. Prog Biol Sci (2015) 5 (2): 181-193.
[6] Dwivedi SK, Dey S. Medicinal herbs: A potential source of toxic metal exposure for man and animals in India. Arch Environ Health (2002) 57 (3): 229-231.
[7] Steenkamp V, Stewart MJ, Curowska E, Zuckerman M. A severe case of multiple metals poisoning in a child treated with a traditional medicine. Forensic Sci Int (2002)128(3): 123-126.
[8] World Health Organization: WHO Guidelines for assessing quality of herbal medicines with reference to contaminants and residues. WHO Publishers: Geneva (2007).
[9] Ajasa A, Bello MO, Ibrahim AO, Ogunwande IA, Olawore NO. Heavy trace metals and macronutrients status in herbal plants of Nigeria. Food Chem (2004) 85 (1): 67-71.
[10] Desideri D, Meli MA, Roselli C. Determination of essential and non-essential elements in some medicinal plants by polarized X ray fluorescence spectrometer (EDPXRF). Microchem J (2010) 95 (2): 174-180.
[11] Gajalakshmi S, Iswarya V, Ashwini R, Divya G, Mythili S, Sathiavelu A. Evaluation of heavy metals in medicinal plants growing in Vellore District. Eur J Exp Biol (2012) 2 (5): 1457-1461.
[12] Debnath M, Khandelwal M, Lal P, Jain R. Evaluation of heavy metal distribution and antibacterial activities of medicinal plants Tinospora cordifolia, Ocimum sanctum and Piper nigrum. Int J Pharm Sci Drug Res (2014) 6 (3): 229-234.
[13] Okem A, Southway C, Stirk WA, Street RA, Finnie JF, Van Staden J. Heavy metal contamination in South African medicinal plants: a cause for concern. S Afr J Bot (2014) 93: 125-130.
[14] Salehi P, Asghari B, Mohammadi F. Biosorption of Ni(II), Cu(II) and Pb(II) by Punica granatum from aqueous solutions. J Water Resour Prot (2010) 2 (8): 701-705.
[15] Street RA, Kulkarni MG, Stirk WA. Southway C, Van Staden J. Variation in heavy metals and microelements in South African medicinal plants obtained from street markets. Food Addit Contam (2008) 25 (8): 953-960.
[16] Khan H, Khan MA, Mahmood T, Choudhary MI. Antimicrobial activity of Gloriosa superba L. (Colchicaceas) extracts. J Enzyme Inhib Med Chem (2008) 23 (6): 855-859.
[17] Rascio N, Navari-Izzo F. Heavy metal hyperaccumulating plants: How and why do they do it? And what makes them so interesting? Plant Sci (2011) 180(2): 169-181.
[18] Okem A, Southway C, Stirk WA, Street RA, Finnie JF, Van Staden J. Effect of cadmium and aluminum on growth, metabolite content and biological activity in Drimia elata (Jacq.) Hyacinthaceae. S Afr J Bot (2015) 98: 142-147.
[19] Akinpelu DA, Aiyegoro OA, Okoh AI. The in vitro antioxidant property of methanolic extract of Afzelia africana (Smith.). J Med Plants Res (2010) 4 (19): 2021-2027.
[20] Kokanova-Nedialkova Z, Nedialkov PT, Nikolov SD. The genus Chenopodium: Phytochemistry ethnopharmacology and pharmacology. Pharmacogn Rev (2009) 3 (6): 280-306.
[21] El-Amier YA, Abdelghany AM, Abed Zaid A. Green synthesis and antimicrobial activity of Senecio glaucus - mediated silver nanoparticles. Res J Pharm Biol Chem Sci (2014) 5 (5): 631-642.
[22] Oladipupo LA, Adebola OO. Chemical composition of the essential oils of the flowers, leaves and stems of two Senecio polyanthemoides Sch. Bip. samples from South Africa. Molecules (2009) 14 (6): 2077-2086.
[23] Gasparetto JC, Martins CAF, Hayashi SS, Otuky MF, Pontarolo R. Ethnobotanical and scientific aspects of Malva sylvestris L.: a millennial herbal medicine. J Pharm Pharmacol (2011) 64 (2):172-189.
[24] Samavati V, Manoochehrizade A. Polysaccharide extraction from Malva sylvestris and its anti-oxidant activity. Int J Bio Macromol (2013) 60: 427-436.
[25] Jimenez-Arellanes A, Meckes M, Ramirez R, Torres J, Luna-Herrera J. Activity against multidrug-resistant Mycobacterium tuberculosis in Mexican plants used to treat respiratory diseases. Phytother Res (2013) 17 (8): 903-908.
[26] Paolini J, Barboni T, Desjobert JM, Djabou N, Muselli A, Costa J. Chemical composition, interspecies variation and seasonal variation in essential oils of Calendula arvensis L. Biochem Syst Ecol (2010) 38 (5): 865-874.
[27] Asadi-Samani M, Kafash-Farkhad N, Azimi N, Fasihi A, Alinia-Ahandani E, Rafieian-Kopaei M. Medicinal plants with hepatoprotective activity in Iranian folk medicine. Asian Pac J Trop Biomed (2015) 5 (2): 146-157.
[28] Erctin T, Senol FS, Orhan IE, Toker G. Comparative assessment of antioxidant and cholinesterase inhibitory properties of the marigold extracts from Calendula arvensis L. and Calendula officinalis L. Ind Crop Prod (2012) 36 (1): 203-208.
[29] Motamedi H, Seyyednejad SM, Hasannejad Z, Dehghani F. Comparative study on the effects of Ziziphus Spina-christi alcoholic extracts on growth and structural integrity of bacterial pathogens. Iran J Pharm Sci (2014)10 (2): 1-10.
[30] Kovacs B, Gyori Z, Prokisch J, Loch J, Daniel, P. A study of plant sample preparation and Inductively Coupled Plasma Emission Spectrometry parameters. Commun Soil Sci Plan (1996) 27 (5-8): 1177-1198.
[31] Soon YK, Abboud S. Cadmium, Chromium, Lead and Nickel. Carter MR (Ed.) In: Soil sampling and methods of analysis, Lewis Publishers CRC, Boca Raton (1993) 101-109.
[32] Hussain Z, Mohammed Khan K, Perveen S, Ambreeen N, Ur Rahman W, Ullah A. The effect of cadmium and chromium concentration, on biological activity of Marsilea minuta. J Chem Soc Pak (2011) 33 (6): 874-876.
[33] Hansch R, Mendel RR. Physiological function of mineral micronutrients (Cu, Zn, Mn, Fe, Ni, Mo, B, Cl). Curr Opin Plant Biol (2009)12 (3): 259-266.
[34] Choy CMY, Lam CW, Cheung LT, Briton-Jones CM, Cheung LP, Haines CJ. Infertility, blood mercury concentrations and dietary seafood consumption: A case-control study. Brit J Obstet Gynaec (2002) 109 (10): 1121-1125.
[35] Street RA, Kulkarni MG, Stirk WA, Southway C, Abdillahi HS, Chinsamy M, Van Staden J. Effect of cadmium uptake and accumulation on growth and antibacterial activity of Merwilla pumbea - an extensively used medicinal plant in South Africa. S Afr J Bot (2009) 75: 611–616.
[36] Noori M, Malayeri B, Moosaei M, Pakzad R, Piriye MH. Effects of heavy metals on the antibacterial properties of Verbascum speciosum Schard. Revista Cientifica UDO. Agrícola (2012) 12 (2): 463–471.
[37] Dorman HJD, Deans SG. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol (2000) 88: 308-316.
[38] Michalak A. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Pol J Environ Stud (2006)15 (4): 523-530.
[39] Marquez-Garcia B, Fernandez-Recamales MA, Cordoba F. Effects of cadmium on phenolic composition and antioxidant activities of Erica andevalensis. J Bot (2012) 2012: 1-6.
[40] Maksimovic ZA, Mravoic M, Dordevic S, Mraovic M. Antimicrobial activity of Chenopodium botrys essential oil. Fitoterapia (2005)76 (1): 112-114.
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