Determination of Anti-melanogenic Activity of Phlomis kurdica in Human Melanoma SKMEL-3 Cells Anti-melanogenic activity of Phlomis kurdica in human melanoma SKMEL-3 cells
Iranian Journal of Pharmaceutical Sciences,
Vol. 12 No. 1 (2016),
15 January 2016
,
Page 1-10
https://doi.org/10.22037/ijps.v12.40781
Abstract
The present study was designed to investigate the anti-melanogenic and cytotoxic activities of methanol extract of Phlomis kurdica. The antioxidant and anti-tyrosinase activity of MeOH extract from P. kurdica (MPk) were examined by DPPH radical scavenging and mushroom tyrosinase activity assays (in vitro), respectively. Furthermore, the effect of MPk on the melanin content, cellular tyrosinase activity and cytotoxicity was studied on human melanoma SKMEL-3 cells (in vivo). The results showed that the MPk inhibited DPPH radicals and mushroom tyrosinase activity in a dose dependent-manner, but these effects were weaker than positive controls. The extract revealed cytotoxic effect in SKMEL-3 cells at high concentrations (> 0.2 mg/mL). Moreover, at concentration of 0.25 mg/mL, it reduced melanin content and cellular tyrosinase activity about 7% and 28% of control, respectively. These findings suggest that the MPk can be considered as a cytotoxic extract in melanoma skin cancers and exhibited inhibitory effect on melanogenesis process.
- antioxidant
- cytotoxicity
- melanin
- Phlomis kurdica
- SKMEL-3 cells
- tyrosinase
How to Cite
References
[2] Solano F, Briganti S, Picardo M, Ghanem GH.Hypopigmenting agents: an updated review on biological, chemical and clinical aspects. Pigment Cell Res (2006) 19(6): 550–571.
[3] Kim YJ, Uyama H. Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future. Cell Mol Life Sci (2005) 62(15): 1707-1723.
[4] Bonnet MF, Mishellany J, Papon A, Cayre F, Penault-Llorca JC, et al. Anti-melanoma efficacy of internal radionuclide therapy in relation with melanin target distribution. Pigment Cell Melanoma Res (2010)
23(5): e1-11.
[5] Sarkhail P, Nikan M, Sarkheil P, Gohari AR, Ajani Y, et al. Quantification of verbascoside in medicinal species of Phlomis and their genetic relationships. Daru (2014) 22(1): 22-32.
[6] Limem-Ben Amor I, Boubaker J, Ben Sgaier M, I.Skandrani, Bhouri W, et al.. Phytochemistry and biological activities of Phlomis species. J Ethnopharmacol (2009) 125(2): 183-202.
[7] Bader A, Tuccinardi T, Granchi C, Martinelli A, Macchia M, et al. Phenylpropanoids and flavonoids from Phlomis kurdica as inhibitors of human lactate dehydrogenase. Phytochemistry, (2015) 116, 262-268.
[8] Limem-Ben Amor I, Boubaker J, Ben Sgaier M, Skandrani I, Bhouri W, Neffati A, Kilani S, Bouhlel I, Ghedira K, Chekir-Ghedira L. Phytochemistry and biological activities of Phlomis species. J Ethnopharmacol (2009) 125(2): 183-202.
[9] Boissy RE, Manga P. On the etiology of contact/occupational vitiligo. Pigment Cell Res (2004) 17(3): 208-214.
[10] Sapkota K, Park SE, Kim JE, Kim S, Choi HS, Chun HS, Kim SJ. Antioxidant and antimelanogenic properties of chestnut flower extract. Biosci Biotechnol Biochem (2010) 74(8): 1527-1533.
[11] Blois MS. Antioxidant determinations by the use of a stable free radical. Nature (1958) 181, 1199-1200.
[12] Sarkhail P, Sarkheil P, Khalighi-Sigaroodi F, Shafiee A, Ostad N. Tyrosinase inhibitor and radical scavenger fractions and isolated compounds from aerial parts of Peucedanum knappii Bornm. Nat Prod Res
(2013) 27(10): 896-899.
[13] Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods (1983) 65(1-2): 55-63.
[14] Chan YY, Kim KH, Cheah SH. Inhibitory effects of Sargassum polycystum on tyrosinase activity and melanin formation in B16F10 murine melanoma cells. J Ethnopharmacol (2011) 137(3): 1183-1188.
[15] Koleva II, van Beek TA, Linssen JP, de Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal (2002) 13(1): 8-17.
[16] Delazar A, Sabzevari A, Mojarrab M, Nazemiyeh H, Esnaashari S, Nahar L, Razavi SM, Sarker SD. Free radical‐scavenging principles from Phlomis caucasica. J Nat Med (2008) 62(4): 464‐466.
[17] Nakayama J1, Guan XC, Nakashima M, Mashino T, Hori Y. In vitro comparison between mouse B16 and human melanoma cell lines of the expression of ICAM-1 induced by cytokines and/or hyperthermia. J
Dermatol (1997) 24(6): 351-360.
[18] Lajis AF, Hamid M, Ariff AB. Depigmenting effect of Kojic acid esters in hyperpigmented B16F1 melanoma cells. J Biomed Biotechnol (2012) 2012:952452. doi: 10.1155/2012/952452.
[19] Son, YO, Lee SA, Kim SS, Jang YS, Chun JC, Lee JC. Acteoside inhibits melanogenesis in B16F10 cells through ERK activation and tyrosinase down-regulation. J Pharm Pharmacol (2011) 63(10): 1309-1319.
- Abstract Viewed: 319 times
- IJPS_Volume 12_Issue 1_Pages 1-10 Downloaded: 254 times