Development and Validation of a Rapid Derivative Spectrophotometric Method for Determination of Tropicamide in Eye Drops Determination of tropicamide in eye drops
Iranian Journal of Pharmaceutical Sciences,
Vol. 6 No. 3 (2010),
1 July 2010
,
Page 171-178
https://doi.org/10.22037/ijps.v6.41269
Abstract
Tropicamide is an antimuscarinic agent used as eye drops for refractive examinations. The aim of this study was to develop a simple and suitable analytical method for determination of tropicamide in eye drops in the presence of excipients. A zero-crossing third and fourth derivative spectrophotometric method was described for determination of tropicamide in eye drops. The measurements were carried out
at wavelengths of 263.8 and 255.4 nm for third- and fourth-derivative, respectively. The method was found to be linear (r2> 0.999) in the range of 10-100 µg/ml for tropicamide in the presence of excipients. The limit of determination was 10 mg/ml for tropicamide. The method was successfully applied for determination of tropicamide in eye drops without any interference from excipients or need to prior separation before analysis.
Keywords:
- Derivative spectrophotometry
- Eye drop
- Tropicamide
- Validation
- Zerocrossing.
How to Cite
Effat Souri, Massoud Amanlou, Shiva Shahbazi, & Mitra Bayat. (2010). Development and Validation of a Rapid Derivative Spectrophotometric Method for Determination of Tropicamide in Eye Drops: Determination of tropicamide in eye drops. Iranian Journal of Pharmaceutical Sciences, 6(3), 171–178. https://doi.org/10.22037/ijps.v6.41269
References
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[15] Hasan NY, Elkway MA, Elzeany BE, Wagieh NE. Stability indicating methods for the determination of diloxanide furoate. J Pharm Biomed Anal 2002; 28: 187-97.
[16] Karpinska J, Kulikowska M. Simultaneous determination of zinc (II), manganese(II) and iron(II) in pharmaceutical preparations. J Pharm Biomed Anal 2002; 29: 153-8.
[17] Senturk N, Erk SA, Ozkan C, Akay S, Cevheroglu S. Determination of theophylline and ephedrine HCL in tablets by ratio-spectra derivative spectrophotometry and LC. J Pharm Biomed Anal 2002; 29: 291-8.
[18] Trabelsi H, Raouafi F, Limam M, Bouzouita K.Derivative spectrophotometric determination of droperidol in presence of parabens. J Pharm Biomed Anal 2002; 29: 239-45.
[19] Lastra OC, Lemus IG, Sanchez HJ, Perez RF. Development and validation of an UV derivative spectrophotometric determination of Losartan potassium in tablets. J Pharm Biomed Anal 2003;33: 175-80.
[20] Markopoulou CK, Koundourellis JE. Two derivative spectrophotometric methods for the simultaneous determination of lovastatin combined with three antioxidants. J Pharm Biomed Anal 2003; 33: 1163-73.
[21] Souri E, Jalalizadeh H, Farsam H, Ghadiri R,Amanlou M. Simultaneous determination of cyproterone acetate and ethinylestradiol in tablets by derivative spectrophotometry. Chem Pharm Bull 2005; 53: 949-51.
[22] Souri E, Amanlou M, Farsam H, Afshari A. A rapid derivative spectrophotometric method for simultaneous determination of naphazoline and antazoline in eye drops. Chem Pharm Bull 2006; 54: 119-22.
[2] European Phramacopoeia. 4th revision, Directorate for Quality of Medicines of the Council of Europe, Stransbourg, France, 2002; pp.2081-2.
[3] The United States Pharmacopiea 29 and The National Formulary 24, The United States Pharmacopeial Convention, Inc. Rockville, MD, 2006; pp. 2223.
[4] British pharmacopoeia. The Stationery office, London, UK, 2005; pp. 2854-5.
[5] Cappello B, Carmignani G, Iervolino M, La Ratondo MI, Saettone MF. Solubilization of tropicamide by hydroxypropyl-beta-cyclodextrin and water-soluble polymers: in vitro/in vivo studies. Int J Pharm 2001; 213: 75-81.
[6] El-Gezawi S, Omar N, El-Rabbat N, Perrin JH.Analysis of some dosage forms containing pyridine derivatives using a cyclodextrin bonded stationary phase in HPLC. J Pharm Biomed Anal 1988; 6: 393-8.
[7] Galmier MJ, Frasey AM, Meski S, Beyssac E, Petit J, Aiache JM, Lartigue C. High-performance liquid chromatographic determination of phenylephrine and tropicamide in human aqueous humor. Biomed Chromatogr 2000; 14: 202-4.
[8] Amanlou M, Asmardi G, Andalibi PC, Javadi N, Khodadady F, Bahrampour Omrany Z. Determination of tropicamide in pharmaceutical formulations using high-performance liquid chromatography. J hromatogr A 2005; 1088:136-9.
[9] Castro D, Moreno MA, Lastres JL. Firstderivative spectrophotometric and LC determination of nifedipine in Brij 96 based oil/water/oil multiple microemulsions on stability studies. J Pharm Biomed Anal 2001; 26: 563-72.
[10] El-Gindy A, El-Zeany B, Awad T, Shabana MM.Spectrophotometric determination of trifluoperazine HCl and isopropamide iodide in binary mixture using second derivative and second derivative of the ratio spectra methods. J Pharm Biomed Anal 2001; 26: 203-10.
[11] Satana E, Altinay S, Gunden Goger N, Ozkan SA, Senturk Z. Simultaneous determination of valsartan and hydrochlorothiazide in tablets by first-derivative ultraviolet spectrophotometry and LC. J Pharm Biomed Anal 2001; 25: 1009-13.
[12] Vega E, Sola N. Quantitative analysis of metronidazole in intravenous admixture with ciprofloxacin by first derivative spectrophotometry. J Pharm Biomed Anal 2001; 25: 523-30.
[13] El-Ragehy NA, Badawey AM, Elkhateeb SZ.Stability indicating methods for the determination of loratadine in the presence of its degradation product. J Pharm Biomed Anal 2002; 28: 1041-53.
[14] Erk N. Simultaneous determination of dorzolamide HCL and timolol maleate in eye drops by two different spectroscopic methods. J Pharm Biomed Anal 2002; 28: 391-7.
[15] Hasan NY, Elkway MA, Elzeany BE, Wagieh NE. Stability indicating methods for the determination of diloxanide furoate. J Pharm Biomed Anal 2002; 28: 187-97.
[16] Karpinska J, Kulikowska M. Simultaneous determination of zinc (II), manganese(II) and iron(II) in pharmaceutical preparations. J Pharm Biomed Anal 2002; 29: 153-8.
[17] Senturk N, Erk SA, Ozkan C, Akay S, Cevheroglu S. Determination of theophylline and ephedrine HCL in tablets by ratio-spectra derivative spectrophotometry and LC. J Pharm Biomed Anal 2002; 29: 291-8.
[18] Trabelsi H, Raouafi F, Limam M, Bouzouita K.Derivative spectrophotometric determination of droperidol in presence of parabens. J Pharm Biomed Anal 2002; 29: 239-45.
[19] Lastra OC, Lemus IG, Sanchez HJ, Perez RF. Development and validation of an UV derivative spectrophotometric determination of Losartan potassium in tablets. J Pharm Biomed Anal 2003;33: 175-80.
[20] Markopoulou CK, Koundourellis JE. Two derivative spectrophotometric methods for the simultaneous determination of lovastatin combined with three antioxidants. J Pharm Biomed Anal 2003; 33: 1163-73.
[21] Souri E, Jalalizadeh H, Farsam H, Ghadiri R,Amanlou M. Simultaneous determination of cyproterone acetate and ethinylestradiol in tablets by derivative spectrophotometry. Chem Pharm Bull 2005; 53: 949-51.
[22] Souri E, Amanlou M, Farsam H, Afshari A. A rapid derivative spectrophotometric method for simultaneous determination of naphazoline and antazoline in eye drops. Chem Pharm Bull 2006; 54: 119-22.
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