Development of Wound Dressing Hydrogel Based Combination of κ-Carrageenan and Chitosan
Iranian Journal of Pharmaceutical Sciences,
Vol. 20 No. 3 (2024),
22 September 2024
,
Page 203- 214
https://doi.org/10.22037/ijps.v20i3.45061
Abstract
Wound dressing has an essential role in the wound-healing process because it can help accelerate wound healing and prevent infection. One of the wound dressings that needs to be developed is a hydrogel-based wound dressing because hydrogel can retain moisture and absorb accumulated fluid on the wound. Moreover, hydrogel can also be used as a matrix for therapeutic agent delivery. Therefore, this study aimed to develop a hydrogel film-based wound dressing using a combination of κ-carrageenan and chitosan. The optimum formula was determined based on the simplex lattice design. The parameters for optimization included swelling ratio, water vapor transmission rate (WVTR), and film degradation ratio. The optimum hydrogel film formula had a concentration of 1.5% κ-carrageenan and 0.5% chitosan. It exhibited a swelling ratio of 423.68 ± 60.52%, a WVTR of 831.54 ± 63.36 mg/cm2.day, a pH of 5.2 ± 0.27, a tensile strength of 0.0681 ± 0.009 mPa, and a hydrogel film degradation of 66.64 ± 20.96% after three days. These results suggest that the optimum hydrogel film-based combination of κ-carrageenan and chitosan is a promising alternative wound dressing.
- κ-carrageenan
- Chitosan
- Hydrogel
- Simplex lattice design
- Wound dressing
How to Cite
References
Baranoski S, Ayello E. Wound Care Essentials. Lippincott Williams & Wilkins;2020.
Rippon M, Waring M, Bielfeldt S, Brandt M. Cell attachment to adhesive dressings: Qualitative and quantitative analysis. Wounds UK. 2015;11: 45-54.
Martin C, Low WL, Amin MCIM, Radecka I, Raj PP, Kenward, K. Current trends in the development of wound dressings, biomaterials, and devices. Pharm Patent Analyst. 2013;2(3):341–359.
Mayet N, Choonara YE, Kumar P, Tomar LK, Tyagi C, Du Toit LC, and Pillay V. J. A comprehensive review of advanced biopolymeric wound healing systems. Pharm Sci. 2014;103(8):2211–2230.
Fromstein and Dawn J. Development and characterization of fibrin and hyaluronan-coated biodegradable polyurethane films. National Library of Canada;2001.
Dong R and Guo B. Smart wound dressings for wound healing. Nano Today. 2021;41:101290.
Das N. Polymers and Polymeric Composites: A Reference Series. 2019;1433–1472.
Martiny TR, Pacheco BS, Pereira CMP, Mansilla A, Astorga–España MS, Dotto GL, Moraes CC, and Rosa GS. A novel biodegradable film based on κ‐carrageenan activated with olive leaves extract. Food Sci Nutr. 2020;8(7):3147–3156.
Kanber AA and Kadhim EF. Efficacy of locally applie k-carragenan on the healing of thermal burn in rat-experimental model: histological study. J Med Dent Sci Res. 2022;10(9):160–165.
Rupert R, Rodrigues KF, Thien VY, Yong WTL. Carrageenan from Kappaphycus alvarezii (Rhodophyta, Solieriaceae): metabolism, structure, production, and application. Front Plant Sci. 2022;13:859635.
Sahana TG and Rekha PD. Biopolymers: Applications in wound healing and skin tissue engineering. 2018;45: 2857–2867.
Allan GG, Altman LC, Bensinger RE, Ghosh DK, Hirabayashi Y, Neogi AN, Neogi S. Biomedical applications of chitin and chitosan (In: Zikakis, J.P. (Ed), Chitin, Chitosan, and Related Enzymes, Orlando: Academic Press. 1984;119-133.
Webber JL, Namivandi‑Zangeneh R, Drozdek S, Wilk KA, Boyer C, Wong EHH, Bradshaw‑Hajek BH, KrasowskaM, and Beattie DA. Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers. Sci Rep. 2021;11:1690.
Sadiq T, Khalid SH, Khan IU, Mahmood H, and Asghar S. A detailed investigation of the effect of calcium cross-linking and glycerol plasticizing on the physical properties of alginate films. Gels. 2022;8(10):652.
Jung C, Kim SJ, Jang J, Ko JH, Kim D, Ko B, Song YM, Hong SH, & Rho J. Disordered-nanoparticle-based etalon for ultrafast humidity-responsive colorimetric sensors and anti-counterfeiting displays. Sci Adv. 2022;8(10):eabm8598.
Chopra H, Bibi S, Kumar S, Khan MS, Kumar P, Singh I. Preparation and Evaluation of Chitosan/PVA Based Hydrogel Films Loaded with Honey for Wound Healing Application. Gels. 2022;8:111.
Mahdavinia GR, Mosallanezhad A, Soleymani M, Sabzi M. Magnetic- and pH-responsive κ-carrageenan/chitosan complexes for controlled release of methotrexate anticancer drug. Int J Biol Macromol. 2017;97:209–217.
Khabbaz B, Solouk A, and Mirzadeh H. Polyvinyl alcohol/soy protein isolate nanofibrous patch for wound-healing applications. Prog Biomater. 2019;8(3):185–196.
Bahadoran M, Shamloo A, and Nokoorani YD. Development of a polyvinyl alcohol/sodium alginate hydrogel-based scaffold incorporating bFGF-encapsulated microspheres for accelerated wound healing. Sci Rep. 2020;10:7342.
Alam J, Alhoshan M, Shukla AK, Aldalbahi A, Ali FAA, Dass LA, and Muthumareeswaran MR. κ-Carrageenan as a promising pore-former for the preparation of a highly porous polyphenylsulfone membrane. Mater Lett. 2017;204:108–111.
Varghese JS, Chellappa N, and Fathima NN. Gelatin-carrageenan hydrogels: role of pore size distribution on drug delivery process. Colloids Surf B. 2014;113:346–351.
Percival SL, McCarty S, Hunt JA, and Woods EJ. The effects of pH on wound healing, biofilms, and antimicrobial efficacy. Wound Repair Regen. 2014;22(2):174–186.
Ninan N, Forget A, Shastri VP, Voelcker NH, and Blencowe A. Antibacterial and Anti-Inflammatory pH-Responsive Tannic Acid-Carboxylated Agarose Composite Hydrogels for Wound Healing. ACS Appl Mater Interfaces. 2016;8(42):28511–28521.
Sim P, Song Y, Yang GN, Cowin AJ, and Garg S. In Vitro Wound Healing Properties of Novel Acidic Treatment Regimen in Enhancing Metabolic Activity and Migration of Skin Cells. Int J Mol Sci. 2022;23(13):7188.
Kruse CR, Nuutila K, Lee CC, Kiwanuka E, Singh M, Caterson EJ, Eriksson E, and Sørensen JA. Wound repair and regeneration: official publication of the Wound Healing Society [and] the European Tissue Repair Societ. 2015;23(4):456–464.
Sim P, Strudwick XL, Song Y, Cowin AJ, and Garg S. Influence of Acidic pH on Wound Healing In Vivo: A Novel Perspective for Wound Treatment. Int J Mol Sci. 2022;23(21),13655.
Park H, Guo X, Temenoff JS, Tabata Y, Caplan AI, Kasper FK, and Mikos AG. Injectable biodegradable hydrogel composites for rabbit marrow mesenchymal stem cell and growth factor delivery for cartilage tissue engineering. Biomacromolecules. 2009;10(3):541–546.
Ruffo M, Parisi OI, Dattilo M, Patitucci F, Malivindi R, Pezzi V, Tzanov T, and Puoci F. Synthesis and evaluation of wound healing properties of hydro-diab hydrogel loaded with green-synthetized AGNPS: in vitro and in ex vivo studies. Drug Deliv Trans Res. 2022;2(8):1881–1894.
Sakiyama T, Chu CH, Fujii T, and Yano T. Preparation of a Polyelectrolyte Complex Gel and its pH-Dependent Swelling Behavior. J Appl Polym Sci. 1993;50(11):2021–2025.
Papagiannopoulos A, Nikolakis SP, Pamvouxoglou A, and Koutsopoulou E. Physicochemical properties of electrostatically cross-linked carrageenan/chitosan hydrogels and carrageenan/chitosan/Laponite nanocomposite hydrogels. Int J Biol Macromol.2023;225:565–573.
Xu R, Xia H, He W, Li Z, Zhao J, Liu B, Wang Y, Lei Q, Kong Y, Bai Y, Yao Z, Yan R, Li H, Zhan R, Yang S, Luo G, and Wu J. Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement. Sci Rep. 2016;6(1):24596.
Halpern JM, Gormley CA, Keech MA, and Von Recum HA. Thermomechanical Properties, Antibiotic Release, and Bioactivity of a Sterilized Cyclodextrin Drug Delivery System. J Mater Chem B. 2014;2(18):2764–2772.
Gonçalves MM, Carneiro J, Justus B, Espinoza JT, Budel JM, Farago PV, & Paula JPD. Preparation and characterization of a novel antimicrobial film dressing for wound healing application. Brazilian J Pharm Sci. 2020;56:e18784.
Wickert LE, Pomerenke S, Mitchell I, Masters KS, & Kreeger PK. Hierarchy of cellular decisions in collective behavior: Implications for wound healing. Sci Rep.2016;6(1): 20139.
Lu S, Zhang X, Tang Z, Xiao H, Zhang M, Liu K, Chen L, Huang L, Ni Y, & Wu H. Mussel-inspired blue-light-activated cellulose-based adhesive hydrogel with fast gelation, rapid hemostasis and antibacterial property for wound healing. Chem Eng J. 2021;417:129329.
Wu P, Fisher AC, Foo PP, Queen D, and Gaylor JD. In vitro assessment of water vapour transmission of synthetic wound dressings. Biomaterials. 1995;16(3):171–175.
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